Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of NELIN.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of SM22α.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of NELIN in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 611 bp (NELIN) and 312 bp (β-actin). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of NELIN mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of SM22α in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 232 bp (SM22α) and 472 bp (GADPH). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of SM22α mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Frontiers in Psychology

Article Title: Acute Effects of Mental Recovery Strategies After a Mentally Fatiguing Task

doi: 10.3389/fpsyg.2020.558856

Figure Lengend Snippet: Schematic overview of the study design. AX-CPT, AX-continuous performance test; RT, reaction time test; VTS, Vienna Test System; SRSS, Short Recovery and Stress Scale; VAS, Visual Analog Scale; SW, SOMNOwatch plus EEG; MC, manipulation check; PN, powernap; SB, systematic breathing; SB+, systematic breathing plus mental imagery; CC, control condition.

Article Snippet: Cognitive performance was assessed using a simple reaction time test (RT, version S1) of the Vienna Test System (VTS, Schuhfried, Moedling, Austria).

Techniques: Control

Journal: European Journal of Medical Research

Article Title: Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population

doi: 10.1186/s40001-024-01705-x

Figure Lengend Snippet: Detection of α-Syn seeding activity by skin RT-QuIC assay in patients with PD and controls. A RT-QuIC detection of α-Syn seeding activity in the cervical skin homogenates from 4 patients with PD and 4 controls. B RT-QuIC detection of α-Syn seeding activity in the skin homogenates of 30 patients and 28 controls. C Scatter graph of maximal thioflavin T (ThT) fluorescence at 70 h of the α-Syn seeding activity by RT-QuIC assay. D ROC curve and AUC calculation for comparison of α-Syn seeding activity between patients with PD and controls ( P < 0.001)

Article Snippet: Each reaction consisted of 2 μL of skin homogenate (0.245–0.5 μg/μL) as a seed and 98 μL of α-Syn RT-QuIC reaction mixture with six 0.8 mm silica beads (OPS Diagnostics, Lebanon, NJ) per well in a 96-well plate (Nalgene Nunc International, 265301).

Techniques: Activity Assay, Fluorescence, Comparison

Journal: European Journal of Medical Research

Article Title: Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population

doi: 10.1186/s40001-024-01705-x

Figure Lengend Snippet: Detection of fibrillary α-Syn in the RT-QuIC product of PD patients. A , B The structural features of α-Syn aggregates extracted from PD-seeded RT-QuIC reactions were analyzed by employing transmission electron microscopy (TEM) and atomic force microscopy (AFM), which could not be observed in the control samples. C Congo red staining, observed under polarized light, exhibited an apple-green color in PD patients’ samples obtained from the RT-QuIC assay, whereas the control samples without PD showed a negative result. The scale bar represents 200 μm. D Analysis of Dot Blot both T0 and RT-QuIC Reaction in PD and Control Groups: Dot blot analysis was conducted on skin samples from three PD and three controls at both T0 and after RT-QuIC reactions using α-Syn filament conformation-specific antibodies (top panel) and total α-Syn antibodies (bottom panel). Densitometric quantification of α-Syn filament conformation-specific and total α-Syn levels revealed significantly increased levels of α-Syn filaments for the PD group compared to the control group, while total αSyn levels did not differ between groups. Results were analyzed using Student’s two-sample t -test. ** p < 0.01 and not significant (ns) when P > 0.05

Article Snippet: Each reaction consisted of 2 μL of skin homogenate (0.245–0.5 μg/μL) as a seed and 98 μL of α-Syn RT-QuIC reaction mixture with six 0.8 mm silica beads (OPS Diagnostics, Lebanon, NJ) per well in a 96-well plate (Nalgene Nunc International, 265301).

Techniques: Transmission Assay, Electron Microscopy, Microscopy, Staining, Dot Blot

Journal: European Journal of Medical Research

Article Title: Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population

doi: 10.1186/s40001-024-01705-x

Figure Lengend Snippet: Skin RT-QuIC results exhibit no correlation with disease progression. Raw RT-QuIC assay parameters reveal no association with PD duration: longer disease duration ( A , E ), higher Hoehn and Yahr (H&Y) stage ( B , F ), higher Unified Parkinson’s Disease Rating Scale (UPDRS) score ( C , G ), and lower Mini-Mental State Examination (MMSE) score ( D , H ) do not exhibit a higher final ThT fluorescence or longer lag phase

Article Snippet: Each reaction consisted of 2 μL of skin homogenate (0.245–0.5 μg/μL) as a seed and 98 μL of α-Syn RT-QuIC reaction mixture with six 0.8 mm silica beads (OPS Diagnostics, Lebanon, NJ) per well in a 96-well plate (Nalgene Nunc International, 265301).

Techniques: Fluorescence